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- J Immunol. 1993 Mar 1;150(5):2072-80.
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Complete Freund's Adjuvant aka CFA ... induced T cells ... apparently prevent the development and adoptive transfer of diabetes in non-obese diabetic mice.
Department of Immunology, University of Alberta, Edmonton, Canada.
T1 or Insulin-dependent diabetes mellitus is a condition of above average blood glucose concentration (AABGC) that is usually characterized by down-regulation of the quantity and/or mass of insulin-producing beta-cells in the islet of Langerhans of the pancreas.
The condition in non-obese diabetic (NOD) mice can be prevented by a single injection of CFA.
This study explores the cellular basis and the time course of the reduction in AABGC.
Since CFA contains a mycobacterial cell wall that has adjuvant property, the role of mycobacteria in young NOD mice was investigated.
Mice injected with Mycobacterium tuberculosis or Mycobacterium bovis (BCG vaccine) at 4 wk of age were also found to be prevented from developing AABGC.
It was found that prevention of AABGC is only achieved by administration of CFA between 4 and 10 wk of age.
Draining lymph node cells or spleen cells from CFA-treated NOD mice transfer the prevention of AABGC. Adoptive transfer of spleen cells from CFA-treated mice with spleen cells from acutely diabetic mice delayed the induction of any AABGC condition into irradiated recipient mice. CFA-treated old NOD mice were also resistant to passive transfer of any AABGC condition by spleen cells from acutely diabetic mice.
Depletion of the Thy 1.2+ cells or CD4(+)-bearing T cells abrogated the prevention of developing AABGC. However, diabetes can be induced in the 'treated' mice by cyclophosphamide treatment.
It was also found that thymocytes from NOD mice responded only weakly to mitogen Con A. CFA treatment, however, restored the ability of these cells to respond to Con A. Finally, the results apparently suggest that T cells induced after CFA treatment of NOD mice prevent both the induction & effector phases of the AABGC condition.
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